#genomics

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GROM – lightning-fast genome variant detection

Published: 20/10/2017

Current human whole genome sequencing projects produce massive amounts of data, often creating significant computational challenges. Different approaches have been developed for each type of genome va ... Read more

Source: www.rna-seqblog.com
Upcoming webinar – A SMARTer® solution to single-cell human T-cell receptor repertoire profiling

Published: 18/10/2017

In this webinar, Dr. Ankita Das and Dr. Sarah Taylor (Takara Bio USA, Inc.) will present data generated with the SMARTer Human scTCR a/b Profiling Kit (SMARTer human scTCR kit), Takara Bio’s first sin ... Read more

Source: www.rna-seqblog.com
powsimR – power analysis for bulk and single cell RNA-seq experiments

Published: 18/10/2017

Power analysis is essential to optimize the design of RNA-seq experiments and to assess and compare the power to detect differentially expressed genes in RNA-seq data. PowsimR is a flexible tool to si ... Read more

Source: www.rna-seqblog.com

Upcoming RNA-Seq Workshop – Analysis of single cell RNA-seq data

Published: 18/10/2017

Analysis of single cell RNA-seq data 5-9 February 2018 Botanisches Museum, Königin-Luise-Straße 6-8, Berlin Instructors: Dr. Vladimir Kiselev Dr. Tallulah Andrews In recent years single cell RNA-seq ( ... Read more

Source: www.rna-seqblog.com
confFuse – high-confidence fusion gene detection

Published: 17/10/2017

Fusion genes play an important role in the tumorigenesis of many cancers. Next-generation sequencing (NGS) technologies have been successfully applied in fusion gene detection for the last several yea ... Read more

Source: www.rna-seqblog.com
RNA-Seq implicates cellular reprograming in model of Alzheimer’s disease

Published: 17/10/2017

Neuroscientists identify genetic changes in microglia in a mouse model of neurodegeneration and Alzheimer’s disease. Microglia, immune cells that act as the central nervous system’s damage senso ... Read more

Source: www.rna-seqblog.com

Global and targeted approaches to single-cell transcriptome characterization

Published: 16/10/2017

Analysing transcriptomes of cell populations is a standard molecular biology approach to understand how cells function. Recent methodological development has allowed performing similar experiments on ... Read more

Source: www.rna-seqblog.com
Featured RNA-Seq Job – Application Scientist

Published: 16/10/2017

Position Description We are seeking an experienced researcher with strong background in RNA biology to consult on scientific and technical inquiries from our customers. The candidate will be located i ... Read more

Source: www.rna-seqblog.com
LSTrAP – efficiently combining RNA sequencing data into co-expression networks

Published: 12/10/2017

Since experimental elucidation of gene function is often laborious, various in silico methods have been developed to predict gene function of uncharacterized genes. Since functionally related genes ar ... Read more

Source: www.rna-seqblog.com

Single-cell approaches to interrogate the transcriptome and proteome of islet cell types in health and disease

Published: 12/10/2017

Blood glucose levels are tightly controlled by the coordinated actions of hormone-producing endocrine cells that reside in pancreatic islets. Islet cell malfunction underlies diabetes development and ... Read more

Source: www.rna-seqblog.com
XML parsing made easy: is that podcast getting longer?

Published: 12/10/2017

Sometime in 2009, I began listening to a science podcast titled This Week in Virology, or TWiV for short. I thought it was pretty good and listened regularly up until sometime in 2016, when it seemed ... Read more

Source: nsaunders.wordpress.com
Act-Seq – detecting activated cell populations using single-cell rna-seq

Published: 12/10/2017

Single-cell RNA sequencing offers a promising opportunity for probing cell types mediating specific behavioral functions and the underlying molecular programs. However, this has been hampered by a lon ... Read more

Source: www.rna-seqblog.com

A modified RNA-seq method for microbial community and diversity analysis using rRNA

Published: 11/10/2017

RNA-seq-based SSU (small subunit) rRNA (ribosomal RNA) analysis has provided a better understanding of potentially active microbial community within environments. However, for RNA-seq library construc ... Read more

Source: www.rna-seqblog.com
SomaGenics Awarded NIH Funding to Develop NGS Single-Cell Analysis of Small RNAs

Published: 11/10/2017

SomaGenics announced the receipt of a Phase I SBIR grant from the NIH to develop RealSeq®-SC (Single Cell), expected to be the first commercially available library preparation kit for profiling small ... Read more

Source: www.rna-seqblog.com
FORMULATRIX® Present Validated SMART-Seq Protocol for Miniaturized cDNA Synthesis Reactions

Published: 11/10/2017

This validated protocol for miniaturized cDNA synthesis reactions was developed to take the guesswork out of reaction miniaturization so that you can: Conserve precious sequencing reagents Sequence mo ... Read more

Source: www.rna-seqblog.com

Feels like a dry winter – but what does the data say?

Published: 11/10/2017

A reminder that when idle queries pop into your head, the answer can often be found using R + online data. And a brief excursion into accessing the Weather Underground. One interesting aspect of Austr ... Read more

Source: nsaunders.wordpress.com
Single nucleotide variants detection using RNA-Seq is subject to high false positive rates

Published: 10/10/2017

High throughput sequencing technology enables the both the human genome and transcriptome to be screened at the single nucleotide resolution. Tools have been developed to infer single nucleotide varia ... Read more

Source: www.rna-seqblog.com
XBSeq2 – a fast and accurate quantification of differential expression and differential polyadenylation

Published: 10/10/2017

RNA sequencing (RNA-seq) is a high throughput technology that profiles gene expression in a genome-wide manner. RNA-seq has been mainly used for testing differential expression (DE) of transcripts bet ... Read more

Source: www.rna-seqblog.com

Identification of genome-wide non-canonical spliced regions and analysis of biological functions for spliced sequences using Read-Split-Fly

Published: 10/10/2017

It is generally thought that most canonical or non-canonical splicing events involving U2- and U12 spliceosomes occur within nuclear pre-mRNAs. However, the question of whether at least some U12-type ... Read more

Source: www.rna-seqblog.com
voomDDA: discovery of diagnostic biomarkers and classification of RNA-seq data

Published: 9/10/2017

https://peerj.com/articles/3890/ voomDDA: discovery of diagnostic biomarkers and classification of RNA-seq data RNA-Seq is a recent and efficient technique that uses the capabilities of next-generatio ... Read more

Source: www.rna-seqblog.com
Single-cell transcriptomics to explore the immune system in health and disease

Published: 9/10/2017

The immune system varies in cell types, states, and locations. The complex networks, interactions, and responses of immune cells produce diverse cellular ecosystems composed of multiple cell types, ac ... Read more

Source: www.rna-seqblog.com

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